Infectious pancreatic necrosis virus triggers antiviral immune response in rainbow trout red blood cells, despite not being infective

Some fish viruses, such as piscine orthoreovirus and infectious Background salmon anemia virus, target red blood cells (RBCs), replicate inside them and induce an immune response. However, the roles of RBCs in the context of infectious pancreatic necrosis virus (IPNV) infection have not been studied yet. : Ex vivo rainbow trout RBCs were obtained from peripheral blood, Methods Ficoll purified and exposed to IPNV in order to analyze infectivity and immune response using RT-qPCR, immune fluorescence imaging, flow cytometry and western-blotting techniques. : IPNV could not infect RBCs; however, IPNV increased the expression Results of the INF1-related genes , and genes. Moreover, conditioned ifn-1 pkr mx media from IPNV-exposed RBCs conferred protection against IPNV infection in CHSE-214 fish cell line. : Despite not being infected, rainbow trout RBCs could respond to Conclusions IPNV with increased expression of antiviral genes. Fish RBCs could be considered as mediators of the antiviral response and therefore targets of new strategies against fish viral infections. Further research is ongoing to completely understand the molecular mechanism that triggers this antiviral response in rainbow trout RBCs. 1 1 1 1